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Age-related decline in hypocretin (orexin) receptor 2 messenger RNA levels in the mouse brain

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
332
Issue
3
Identifiers
DOI: 10.1016/s0304-3940(02)00953-9
Keywords
  • Sleep
  • Aging
  • Energy Metabolism
  • Gene Expression
  • Real-Time Quantitative Polymerase Chain Reaction Analysis
  • Kinetic Polymerase Chain Reaction
  • Dynorphin
Disciplines
  • Medicine

Abstract

Abstract The hypocretin (Hcrt; also known as orexin) system has been implicated in arousal state regulation and energy metabolism. We hypothesize that age-related sleep problems can result from dysfunction of this system and thus measured messenger RNA (mRNA) levels of preprohcrt in the hypothalamus, and hcrt receptor 1 (hcrtr1) and hcrt receptor 2 (hcrtr2) in eight brain regions of 3, 12, 18 and 24 months old C57BL/6 mice. Expression of preprohcrt and the colocalized prodynorphin did not change with age. Whereas an age-related change in hcrtr1 mRNA expression was observed only in the hippocampus, hcrtr2 mRNA levels declined in the hippocampus, thalamus, pons, and medulla; these reductions ranged from 33 to 44%. Declining trends ( P<0.1) in hcrtr2 mRNA levels were also observed in the cortex, basal forebrain and hypothalamus. These results are consistent with the hypothesis that an age-related deterioration occurs in the Hcrt system that may contribute to age-related sleep disorders.

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