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Purification of T4 RNA ligase by Dextran Blue-Sepharose 4B affinity chromatography

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
125
Issue
1
Identifiers
DOI: 10.1016/0003-2697(82)90375-x
Disciplines
  • Biology

Abstract

Abstract A simplified purification method of the T4 RNA ligase using Dextran Blue-Sepharose 4B chromatography is described. The purified T4 RNA ligase gave one band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 32 pC p and C pG pG pA were incubated with the purified enzyme and subjected to homochromatography. No degradation products were found, and this purified enzyme could be used in the synthesis of oligonucleotides of defined sequence.

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