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Rapid purification of a prekallikrein from rat pancreas

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
113
Issue
2
Identifiers
DOI: 10.1016/0003-2697(81)90076-2

Abstract

Abstract A prekallikrein from rat pancreas was purified 1500-fold with an overall yield of 20% using a rapid, simple procedure. DEAE-Sephadex A-50 chromatography permitted the separation of two prekallikrein components present in rat pancreatic homogenates; the major fraction was further purified by Sephadex G-100 gel filtration and immunoadsorption chromatography. The zymogen is a single-chain molecule with p I 4·35. Apparent M r values of 38,000 and 37,000 were determined by sodium dodecyl sulfate-polyacrylamide gradient electrophoresis and gel filtration, respectively.

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