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Over-Expression of Human Lipoprotein Lipase in Mouse Mammary Glands Leads to Reduction of Milk Triglyceride and Delayed Growth of Suckling Pups

Authors
Publisher
Public Library of Science
Publication Date
Volume
6
Issue
6
Identifiers
DOI: 10.1371/journal.pone.0020895
Keywords
  • Research Article
  • Agriculture
  • Animal Management
  • Animal Breeding
  • Animal Nutrition
  • Transgenic Animals
  • Biology
  • Biochemistry
  • Proteins
  • Lipoproteins
  • Biotechnology
  • Genetic Engineering
  • Transgenics
  • Molecular Cell Biology
  • Cellular Types
  • Endothelial Cells
Disciplines
  • Biology

Abstract

Background The mammary gland is a conserved site of lipoprotein lipase expression across species and lipoprotein lipase attachment to the luminal surface of mammary gland vascular endothelial cells has been implicated in the direction of circulating triglycerides into milk synthesis during lactation. Principal Findings Here we report generation of transgenic mice harboring a human lipoprotein lipase gene driven by a mammary gland-specific promoter. Lipoprotein lipase levels in transgenic milk was raised to 0.16 mg/ml, corresponding to an activity of 8772.95 mU/ml. High lipoprotein lipase activity led to a significant reduction of triglyceride concentration in milk, but other components were largely unchanged. Normal pups fed with transgenic milk showed inferior growth performances compared to those fed with normal milk. Conclusion Our study suggests a possibility to reduce the triglyceride content of cow milk using transgenic technology.

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