DRUGS OF ABUSE IN SERUM BY UHPLC-MSIMS 75 VALI QUANTITATIVE MULTAN DETERMINATION COCAIN AND AMPHETAMINES IN SERUM SV U PLC COUPLED TANDEM MASS N. Dubois 1, B. , Ph. Hubert2,3, C. words: Cocaine, UlJlcllt::>, :,.-nnh",t;:,nnin,:.c UHPLC-MS/MS nn"thnrl validat:ion total errer 1 Service Méclicolégalle, de ('Environnement et en I=ntr'""ri"t> CHU Sart-Tilman, 2 Service de Chimie Analvti~]ue. Département de Pharmacie, Université de 3 CIRM, Université de Address for Corres~)on(jenl:e N. Dubois Service de l'Environnement et en I=ntr'<>l'wi"", CHU Sart-Tilman B35, Tour Il, +5 B-4000 +3243667680 Fax: + 32 4 366 88 89 E-mail: [email protected] The use worldwide of Eu would have used cocaine year (1). Ber1zovLeCQ'clnÎrle is one of the major metabolites of cocaine formed by either spolntëmelDus h\Jl"lrnI1"CIC Acta Clinica 2010; 65-;)uPt)lerrlent 76 DRUGS OF ABUSE IN SERU/I1 BY UHPLC-/I1S//I1S (MBDB) are opioid users is estimated at in Europe andbetween 1.2 650000 of them would have received substitution treatment in 2007. Heroin accounts for the Qreatest share of in the which to very rapidly rnrl\/o"1"or1 matic esterase to form n-;~rptvlr'TInrnhlinp is into morphine esterase. Morphine is quickly to its meJ:aDOtn.e, morphine-3-glucuronide (M3G) somewhat more to amounts mor !'.ln'ICHy-,ho.. use of different Dhlne··o-s'lUl:unClni,de (M6G).According to the report Centre for Drugs 2009 (1), polydrug norm in Europe the com is responsible Cornpl.ICates, most of the problems faces. Knc)wteCH:Je only a few allowed buprenorphine I\laloxone and two narcotic ant:ag()n were quantified. Gas chromatography to mass spectrometry had been widely used for many (7,8) liquid chromato,QraDhv (UHPlC-MS/MS). It reDli:ICed techniques used MATERIALS AND materials for aU compounds and inter .,. ..........."-,,.,, .. were from lGC Url"1.rYlr,,·norn (Molsheim, France). of pu- rity upper than 99%.