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Selective removal of seedling root hairs for studies of theRhizobium-legume symbiosis

Authors
Journal
Journal of Microbiological Methods
0167-7012
Publisher
Elsevier
Publication Date
Volume
4
Issue
2
Identifiers
DOI: 10.1016/0167-7012(85)90024-7
Keywords
  • Medicago Sativa
  • Rhizobium
  • Root Hair
  • Trifolium Repens
  • Trifoliin A
Disciplines
  • Biology

Abstract

Abstract A freeze-fracture method has been developed for the selective removal of root hairs from white clover ( Trifolium repens L.) and alfalfa ( Medicago sativa L.) seedling. This procedure yields sufficient material for analysis of root hair proteins by polyacrylamide gel electrophoresis and can be adapted to study in vivo protein synthesis in these differentiated epiderman cells. Clover root hairs which have been injected by the nitrogen-fixing symbiont, Rhizobium trifolii 0403, are also detached from roots by this process, yielding appropriate material to study root responses to the bacterial symbiont during the infection process.

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