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The timing of the S phase and other nuclear events in yeast meiosis

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
145
Issue
1
Identifiers
DOI: 10.1016/s0014-4827(83)80022-6

Abstract

Summary The length of the premeiotic S phase in individual cells of a homothallic strain of Saccharomyces cerevisiae was determined by pulse-labelling synchronously sporulating cultures with [ 3H]adenine and following changes in the frequency of cells in S by DNA-specific whole cell autoradiography. The average S phase was found to be at least 2–3 times as long as in mitotic diploids, and it was concluded that activation of replication origins in meiosis is considerably staggered. The timing of S in relation to other meiotic milestones was established by counting different nuclear morphologies visualised by DAPI-fluorescent staining. This allowed tentative identification of pachytene nuclei as well as first and second nuclear divisions.

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