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Ultrastructural examination of the squid giant axons perfused intracellularly with protease

Authors
Journal
Journal of Ultrastructure Research
0022-5320
Publisher
Elsevier
Publication Date
Volume
25
Identifiers
DOI: 10.1016/s0022-5320(68)80094-2
Disciplines
  • Biology

Abstract

An electron microscopic study was made on squid giant axons perfused with a kind of protease, BPN' ( Bacillus protease, strain N'). The axon treated with this enzyme maintained excitability for more than 1 hour. After 8 min. of perfusion, practically no axoplasm remained inside the plasma membrane. The protease affected the internal structure of the Schwann cell to some extent, whereas the structure of the plasma membranes appeared to be normal at the level of the electron microscope. These axons can be used for physiological investigation of the fundamental mechanism of excitability.

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