Affordable Access

Publisher Website

Gas challenge–blood oxygen level-dependent (GC-BOLD) MRI in the rat Novikoff hepatoma model

Authors
Journal
Magnetic Resonance Imaging
0730-725X
Publisher
Elsevier
Publication Date
Volume
30
Issue
1
Identifiers
DOI: 10.1016/j.mri.2011.09.007
Keywords
  • Gas Challenge–Blood Oxygen Level Dependent (Gc-Bold)
  • Mri
  • Rat Novikoff Hepatoma
  • Tumor Angiogenesis
  • Tumor Microvessel Density
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Purpose The purpose of the study was to investigate the relationship between gas challenge–blood oxygen level-dependent (GC-BOLD) response angiogenesis and tumor size in rat Novikoff hepatoma model. Materials and Methods Twenty adult male Sprague–Dawley rats (weighting 301–325 g) were used for our Animal Care and Use Committee-approved experiments. N1-S1 Novikoff hepatomas were grown in 14 rats with sizes ranging from 0.42 to 2.81 cm. All experiments were performed at 3.0 T using a custom-built rodent receiver coil. A multiple gradient-echo sequence was used for R2 ⁎ measurements, first during room air (78% N 2/20% O 2) breathing and then after 10 min of carbogen (95% O 2/5% CO 2) breathing. After image acquisition, rats were euthanized, and the tumors were harvested for histological evaluation. Results The R2 ⁎ change between air and carbogen breathing for small hepatomas was positive; R2 ⁎ changes changed to negative values for larger hepatomas. We found a significant positive correlation between tumor R2 ⁎ change and tumor microvessel density (MVD) ( r=0.798, P=.001) and a significant inverse correlation between tumor R2 ⁎ change and tumor size ( r=−0.840, P<.0001). Conclusions GC-BOLD magnetic resonance imaging measurements are well correlated to MVD levels and tumor size in the N1-S1 Novikoff hepatoma model; GC-BOLD measurements may serve as noninvasive biomarkers for evaluating angiogenesis and disease progression and/or therapy response.

There are no comments yet on this publication. Be the first to share your thoughts.