Abstract A comparative evaluation of the enzyme-linked immunosorbent assay (Elisa), passive haemagglutination (PHA) and counterimmunoelectrophoresis (CIE) methods were carried out. Approx. 6% of control samples were positive with the Elisa assay, while the values for Behring Institute (BI) passive haemagglutination, Wellcome Research Laboratory (WRL) passive haemagglutination and CIE were 2.7%, 2.2% and 3.3%, respectively. The above data contrast with values of 21.3%, 10.6, 10.3 and 12.3 obtained in sera from chronic glomerulonephritis patients. Our observation suggests that HbsAg may be associated with chronic glomerulonephritis.