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Non-processive transcription of poly[d(A—T)] by wheat germ RNA polymerase II

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
150
Issue
2
Identifiers
DOI: 10.1016/0014-5793(82)80793-x
Keywords
  • Eukaryotic Rna Polymerase
  • Poly[D(A—T)]Transcription
  • Kinetic Processivity
Disciplines
  • Biology

Abstract

Abstract RNA product distribution obtained during the transcription of poly[d(A—T)] by wheat germ RNA polymerase IIA under various experimental conditions was analyzed by high resolution polyacrylamide gel electrophoresis. Poly[r(A—U)] synthesis proceeded as if wheat germ RNA polymerase II was a non-processive enzyme: a ladder of RNA products of increasing lengths was obtained, which apparently, terminated at every other nucleotide. RNA release was not dependent upon nucleoside triphosphate substrate concentrations. A likely explanation would be that ternary complexes enzyme: DNA: RNA were very much unstable; moreover, oligonucleotides released were not re-used for further elongation by the enzyme.

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