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Detection of heparan sulfate in spinal spheroids of β,β′-iminodipropionitrile (IDPN)-treted rats

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
202
Identifiers
DOI: 10.1016/0304-3940(95)12225-7
Keywords
  • β
  • β′-Iminodipropionitrile (Idpn)
  • Heparan Sulfate
  • Axonal Swellings
  • Spheroids
  • Amyotrophic Lateral Sclerosis
  • Neurofilaments
  • Spinal Cord
Disciplines
  • Chemistry

Abstract

Abstract β,β′-Iminodipropionirtrile (IDPN) is known to produce a massive accumulation of neurofilaments in the proximal portion of axons of spinal anterior horn cells. The spinal cords of 20 Wistar rats treated with IDPN were examined immunohistochemically with a monoclonal antibody against heparan sulfate (HepSS-1). Virtually all axonal swellings were intensely labeled with HepSS-1. This immunoreactivity was almost completely absorbed by the presence of CDSNS-HS (completely desulfated, N-sulfated heparan sulfate). Western blot study revealed that HepSS-1 recognized four distinct bands at the positions of ∼17 kDa, ∼20 kDa, ∼21 kDa, and ∼25 kDa. The present study suggests that the deposit of heparan sulfate in spheroids is related to the pathomechanism for the formation of axonal swelling.

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