Affordable Access

Publisher Website

Parathyroid hormone-related peptide and Indian hedgehog expression patterns in naturally acquired equine osteochondrosis

Authors
Journal
Journal of Orthopaedic Research
0736-0266
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
20
Issue
6
Identifiers
DOI: 10.1016/s0736-0266(02)00055-4
Keywords
  • Dyschondroplasia
  • Osteochondrosis
  • Pth-Rp
  • Ihh
  • Development
Disciplines
  • Biology
  • Chemistry
  • Medicine

Abstract

Abstract Early changes in parathyroid hormone-related peptide (PTH-rP) and Indian hedgehog (Ihh) expression were examined in equine articular osteochondrosis (OC) as a model of a naturally acquired dyschondroplasia. Cartilage was harvested from OC-affected femoropatellar or scapulohumeral joints from immature horses and normal control horses of similar age. PTH-rP expression levels were assessed by semi-quantitative PCR, in situ hybridization, and immunohistochemistry. Ihh protein expression levels were assessed by immunohistochemistry. Elevated PTH-rP protein and mRNA expression were identified in the deeper layers of affected articular cartilage and the fibrous tissue of interposing clefts. These changes were confined to the chondrocytes in the OC-affected cartilage, which had significantly increased PTH-rP protein and mRNA expression when compared to control cartilages. Ihh protein expression showed similar distribution as PTH-rP in the deeper layers of articular cartilage; however, only a trend for increased Ihh immunostaining was evident in the OC cartilage when compared to the normal cartilage. Increased PTH-rP expression in pre-hypertrophic chondrocytes of diseased OC cartilage suggests a possible link between this peptide and the delayed ossification, which is a consistent histologic alteration in OC. More evidence is necessary to determine the role of Ihh in articular cartilage and if a similar feedback cycle exists as previously described for the growth plate.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Parathyroid hormone-related peptide and indian hed...

on Journal of orthopaedic researc... November 2002

Expression patterns of hedgehog signaling peptides...

on Journal of orthopaedic researc... September 2005

Expression patterns of hedgehog signaling peptides...

on Journal of Orthopaedic Researc... Jan 01, 2005

Parathyroid hormone-related peptide and Indian hed...

on Current opinion in nephrology... July 2000
More articles like this..