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The influence of albumin on vitamin D metabolism in fetal chick osteoblast-like cells

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
125
Issue
1
Identifiers
DOI: 10.1016/s0006-291x(84)80363-0

Abstract

Summary Incubation of osteoblast-like cells with [3H]25-(OH)D3 and varying bovine serum albumin (BSA) concentrations resulted in a dramatic change in the accumulation of 1,25-(OH)2D3 and 24,25-(OH)2D3 in the medium. At 0.1% BSA 1,25-(OH)2D3 formation was transient and 24,25-(OH)2D3 was the main product after 3 h. At 2% BSA accumulation of 1,25-(OH)2D3 was sustained whereas 24,25-(OH)2D3 formation was suppressed. At low BSA levels added [3H]1,25-(OH)2D3 was rapidly metabolized to 1,24,25-(OH)3D3 and more polar metabolites. The effect of increasing BSA concentrations on 25-(OH)D3 metabolism was mimicked by addition of cycloheximide. This indicates that high BSA levels prevent the induction of 24-hydroxylase activity in this system, probably by lowering of the free 25-(OH)D3 concentration. The accumulation of 1,25-(OH)2D3 from 25-(OH)D3 not only depends on the 1α-hydroxylase activity, but also on the further metabolism of 1,25-(OH)2D3 by 24-hydroxylation.

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