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The T-cell-independent immune response to the hapten NP uses a large repertoire of heavy chain genes

Authors
Journal
Cell
0092-8674
Publisher
Elsevier
Publication Date
Volume
43
Issue
3
Identifiers
DOI: 10.1016/0092-8674(85)90244-2

Abstract

Abstract Hybridomas generated from C57BL/6 mice immunized with the hapten NP coupled to ficoll, a T-cell-independent carrier, produce monoclonal antibodies that use a large repertoire of V H regions and light chains. This contrasts with the homogeneity of the strain-specific response to NP observed with T-cell-dependent carriers, where most of the antibodies use a single V H region, V186.2, in combination with the lambda-1 light chain. There is no evidence for somatic mutation in any of the sequenced regions of the antibodies generated by NP-ficoll. Thus T cell participation is required for the homogeneity of the strain-specific hapten response, and probably for somatic mutation as well.

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