Affordable Access

Publisher Website

One-step RT-PCR for detection of Zika virus

Authors
Journal
Journal of Clinical Virology
1386-6532
Publisher
Elsevier
Publication Date
Volume
43
Issue
1
Identifiers
DOI: 10.1016/j.jcv.2008.05.005
Keywords
  • Zika Virus
  • Detection
  • Diagnosis
  • Rt-Pcr
Disciplines
  • Biology
  • Design
  • Medicine

Abstract

Abstract Background Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. Objective To develop a one-step RT-PCR assay to detect ZIKV in human serum. Study design An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. Results The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. Conclusions The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

[Hepatitis delta virus RNA detection by one-step R...

on Annales de biologie clinique 2004

Rapid and quantitative detection of mumps virus RN...

on Diagnostic Microbiology and In... June 2004

Detection ofTomato black ring virusby real-time on...

on Journal of Virological Methods Jan 01, 2011

Rapid and quantitative detection of mumps virus RN...

on Diagnostic Microbiology and In... Jan 01, 2004
More articles like this..