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Extracellular ATP and P2 receptors are required for IL-8 to induce neutrophil migration

Authors
Publisher
Elsevier Ltd
Publication Date
Volume
46
Issue
2
Identifiers
DOI: 10.1016/j.cyto.2009.02.011
Keywords
  • Chemokine
  • Chemotaxis
  • Inflammation
  • Fmlp
  • Extracellular Nucleotides

Abstract

Abstract The chemokine interleukin 8 (IL-8) is a major chemoattractant for human neutrophils. Here, we demonstrate novel evidence that IL-8-induced neutrophil chemotaxis requires a concurrent activation of P2 receptors, most likely the P2Y 2 which is dominantly expressed in these cells. Indeed, the migration of human neutrophils towards IL-8 was significantly inhibited by the P2Y receptor antagonists, suramin and reactive blue 2 (RB-2) and potentiated by a P2Y 2 ligand, ATP, but insensitive to specific antagonists of P2Y 1, P2Y 6 and P2Y 11 receptors. Adenosine had no effect on neutrophil migration towards IL-8 which contrasted with the stimulatory effect of this molecule on neutrophil chemotaxis caused by formyl-Met-Leu-Phe (fMLP or fMLF). Taken together, these data suggest that extracellular ATP is necessary for IL-8 to exert its chemotactic effect on neutrophils.

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