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[56] Carbodiimide modification of proteins

Authors
  • Carraway, K.L.
  • Koshland, D.E. Jr.
Type
Book
Journal
G Protein Coupled Receptors - Structure
Publisher
Elsevier BV
Publication Date
Jan 01, 1972
Volume
25
Pages
616–623
Identifiers
DOI: 10.1016/S0076-6879(72)25060-1
ISBN: 978-0-12-181888-3
Source
Elsevier
License
Unknown

Abstract

A carbodiimide-nucleophile procedure for modifying carboxyl groups in proteins can be utilized under mild conditions for the quantitative determination of all carboxyl groups or for the modification of selected residues in activity studies. The method allows numerous variations both in the activating carbodiimide and in the nature of the nucleophile. Side reactions do occur but can be corrected for.

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