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Increase of cloning efficiencies by using high-performance liquid chromatography-purified vectors linkers

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
296
Identifiers
DOI: 10.1016/s0021-9673(01)96432-5

Abstract

Abstract High-performance liquid chromatography (HPLC) was applied to the purification of vector linker DNAs used in molecular cloning experiments. The data clearly demonstrate that HPLC (Blue Column from LKB/TSK G 4000 SW) based on steric exclusion is a very rapid convenient method for the size separation of DNA fragments that are virtually free of any contamination. Application of vector and linker DNAs purified by this method enabled us to clone nanogram amounts of avian myeloblastosis virus RNA which was used for the evaluation of a recently described cloning procedure.

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