Affordable Access

Publisher Website

The Frequency and Position ofAluRepeats in cDNAs, as Determined by Database Searching

Authors
Journal
Genomics
0888-7543
Publisher
Elsevier
Publication Date
Volume
27
Issue
3
Identifiers
DOI: 10.1006/geno.1995.1090
Disciplines
  • Biology

Abstract

Abstract The Alu repeat sequence is estimated to account for 5% of human genomic DNA. The precise relationship of Alu sequences to human fully spliced cDNA has yet to be determined, although many new protocols for cloning cDNAs either depend on the presence of Alus or—more usually—rely on their absence in a population of messages. Previous estimates of the percentage of fully spliced human transcripts that contain Alu repeats have relied on hybridization procedures. Here we have gone directly to the DNA sequence by extracting over 1600 entries from GenBank that are described as human complete cDNAs, and we have assessed the frequency with which the Alu repeat sequence occurs in these sequences. We find that 5% of fully spliced human cDNAs contain Alu sequences. In addition, we have quantified the appearance of Alus in the different cDNA regions, 5′ untranslated region (UTR), coding region, and 3′ UTR. The vast majority of Alus are found in the 3′ UTR, but 14% lie in the 5′ UTR, and very rarely an Alu sequence is present within, or partially within, the coding region of the transcript.

There are no comments yet on this publication. Be the first to share your thoughts.