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Structure of the O-polysaccharide from the LPS of aHafnia alveistrain isolated from a patient with suspect yersinosis

Authors
Journal
Carbohydrate Research
0008-6215
Publisher
Elsevier
Publication Date
Volume
300
Issue
2
Identifiers
DOI: 10.1016/s0008-6215(97)00045-1
Keywords
  • Hafnia Alvei
  • Nmr Spectroscopy
  • Lipopolysaccharide
  • Structure

Abstract

Abstract The carbohydrate backbone of the Hafnia alvei strain Y166/91 lipopolysaccharide (LPS) was isolated by mild acid hydrolysis followed by gel permeation chromatography and studied by NMR spectroscopy and methylation analysis. Treatment with periodate and hypoiodite gave a modified polysaccharide which was also characterised. It was concluded that the polysaccharide has the following structure with two distinct regions, which are connected. The chain length parameters m and n were not determined but the ratio m/n is approximately 2. [→ 3)-β- d-Gal p-(1 → 3)-α- d-Gal p-(1 → 3)-β- d-Gal p-(1 → 3)-β- d-Gal f-(1 →] n From the present data it is not possible to determine whether it is the Gal p-Gal p chain or the Gal p-Gal f chain that is connected to the core. The structure found here is identical to that suggested for the O-specific polysaccharide chain of Klebsiella pneumoniae O1K2 (NCTC 5055) LPS [O. Kol, J.-M. Wieruszeski, G. Strecker, J. Montreuil, and B. Fournet, Carbohydr. Res., 217 (1991) 117–125; O. Kol, J.-M. Wieruszeski, G. Strecker, B. Fournet, R. Zalisz, and P. Smets, Carbohydr. Res., 236 (1992) 339–344].

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