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A vector for the expression of recombinant monoclonal Fab fragments in bacteria

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
217
Identifiers
DOI: 10.1016/s0022-1759(98)00102-1
Keywords
  • Monoclonal Recombinant Antibodies
  • Combinatorial Libraries
Disciplines
  • Mathematics

Abstract

Abstract The availability of genes coding for monoclonal Fab fragments of a desired specificity permits their expression in bacteria and provides a simple method for the generation of good quality reagents. In this paper we describe a new phagemid vector for the production of recombinant Fabs from genes obtained from phage display combinatorial libraries. The phagemid features an antibiotic resistance cassette which, once inserted between the heavy chain fragment and the light chain genes, avoids unwanted recombination and preserves useful restriction sites not affecting the Fab production rate.

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