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Ethylene Rapidly Up-Regulates the Activities of Both Monomeric GTP-Binding Proteins and Protein Kinase(s) in Epicotyls of Pea1

Authors
Publisher
American Society of Plant Biologists
Publication Date
Source
PMC
Keywords
  • Research Article
Disciplines
  • Biology

Abstract

Ethylene Rapidly Up-Regulates the Activities of Both Monomeric GTP-Binding Proteins and Protein Kinase(s) in Epicotyls of Pea by Igor E. Moshkov, Galina V. Novikova, Luis A.J. Mur, Aileen R. Smith, and Michael A. Hall Plantphysiol Volume 131(4):1718-1726 April 1, 2003 ©2003 by American Society of Plant Biologists Time course of GTP binding to monomeric G-proteins extracted with 750 mm KCl (A) and 1% (w/v) Triton X-100 (B) as affected by ethylene (1 μL L−1, ●), MCP (100 nL L−1, ○) and when ethylene was applied after pretreatment with MCP (▾). Moshkov I et al. Plantphysiol 2003;131:1718-1726 ©2003 by American Society of Plant Biologists Two-dimensional separations of G-proteins. Moshkov I et al. Plantphysiol 2003;131:1718-1726 ©2003 by American Society of Plant Biologists Two-dimensional separations of G-proteins. Moshkov I et al. Plantphysiol 2003;131:1718-1726 ©2003 by American Society of Plant Biologists Immunoprecipitation of [α-32P]GTP-labeled monomeric G-proteins with anti-Rab 8 antibodies. Moshkov I et al. Plantphysiol 2003;131:1718-1726 ©2003 by American Society of Plant Biologists MAP kinase activity in pea epicotyls as affected by ethylene, MCP, and NBD. Pea seedlings were treated with ethylene (1 μL L−1, 20 min), MCP (100 nL L−1, 2 h), NBD (2,000 μL L−1, 2 h), or inhibitors of ethylene binding (as indicated above) followed by ethyl... Moshkov I et al. Plantphysiol 2003;131:1718-1726 ©2003 by American Society of Plant Biologists Time course of ethylene-modulated MAP kinase activity. Moshkov I et al. Plantphysiol 2003;131:1718-1726 ©2003 by American Society of Plant Biologists Time course of GTP binding to monomeric G-proteins extracted with 750 mm KCl (A) and 1% (w/v) Triton X-100 (B) as affected by ethylene (1 μL L−1, ●), MCP (100 nL L−1, ○) and when ethylene was applied after pretreatment with MCP (▾). The arrow indicates time point of ethylene application. Experimental points are derived from scans of one-dimensional autoradiographs of SDS-PAGE separations of labe

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