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A kinetoplast DNA minicircle probe (B18) specific for theLeishmaniasubgenusViannia

Authors
Journal
Transactions of the Royal Society of Tropical Medicine and Hygiene
0035-9203
Publisher
Oxford University Press
Publication Date
Volume
96
Identifiers
DOI: 10.1016/s0035-9203(02)90054-4
Keywords
  • Basic Molecular Tools

Abstract

Abstract This paper reports the development, sequence and specificity of probe B18, which hybridizes only to the kinetoplast deoxyribonucleic acid (kDNA) minicircle of Leishmania species of the subgenus Viannia. This probe was developed in 1985 and has been used extensively since, on whole kDNA, Leishmania dot-blots and kDNA minicircles amplified by the polymerase chain reaction.

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