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An evaluation of the double thymidine block for synchronizing mammalian cells at the G1-S border

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
68
Issue
1
Identifiers
DOI: 10.1016/0014-4827(71)90599-4

Abstract

Abstract The effect of a double 2 mM thymidine block on L cells and CHOP cells has been investigated. The S phase following release from the second 2 mM thymidine block is about 5 h which represents a shortening by about one-third. Unlabeled mitoses are observed in the first hours after release of the block and labeled mitoses are observed 5 h after the block, 32P-orthophosphate is incorporated in the DNA during the thymidine block at about one-third the rate for non-blocked, control cells. The results indicate that cells continue to synthesize DNA during the thymidine block at about one-third the normal rate and do not accumulate at the G 1-S border.

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