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Interproton distance measurements in solution for a double-stranded DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in thegaloperon:A nuclear Overhauser enhancement study

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
175
Issue
1
Identifiers
DOI: 10.1016/0014-5793(84)80582-7
Keywords
  • Dna Oligonucleotide
  • Noe
  • Interproton Distance
  • Solution Structure
  • Crp Specific Site
Disciplines
  • Biology

Abstract

Abstract Using the proton-proton nuclear Overhauser effect, a large number (150) of interproton distances, both intra- and internucleotide, are determined in solution for a non-self-complementary DNA undecamer comprising a portion of the specific target site for the cAMP receptor protein in the gal operon. It is shown that these distances are very similar to those expected for classical B DNA (RMS difference of 0.5 Å) but are significantly different from those expected for classical A DNA (RMS difference 1.1Å). Glycosidic (χ) and C4'-C3' (δ) bond torsion angles are obtained by model building on the basis of the intranucleotide interproton distances. Whereas the sugar pucker exhibits little base-to-base variation with δ lying in the range 120 ± 10°, the glycosidic bond torsion angles of the pyrimidine and purine residues are significantly different, with χ pyr = −120 ± 10° and χ pur = − 90 ± 10°.

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