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Expression of a carrot invertase gene in tobacco suspension cells cultivated in batch and continuous culture conditions

Authors
Publisher
Elsevier B.V.
Publication Date
Volume
87
Issue
3
Identifiers
DOI: 10.1016/s1389-1723(99)80036-x
Keywords
  • Invertase
  • Transgene Expression
  • Recombinant Protein
  • Plant Cell Culture
  • Nicotiana Tabacum
Disciplines
  • Biology

Abstract

Abstract Plant cells ( Nicotiana tabacum) were genetically modified to produce an heterologous protein, the acidic invertase from carrot, and invertase production from suspension tobacco cells was investigated. Suspension cultures were grown in shake flasks and stirred bioreactor. Total invertase activity was growth related. A 75 d continuous culture in 10 l bioreactor was performed. Our study demonstrates the high potential of plant cell cultures for long term production of heterologous protein.

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