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Resolution of two fractions of androgen receptor from mouse kidney

Authors
Journal
Journal of Steroid Biochemistry
0022-4731
Publisher
Elsevier
Publication Date
Volume
17
Issue
4
Identifiers
DOI: 10.1016/0022-4731(82)90636-7

Abstract

Abstract Putative androgen receptors from mouse kidney were separated and re-chromatographed on DNA-cellulose columns. With either [ 3H]-dihydrotestosterone or [ 3H]-testosterone as ligands, two major fractions of androgen receptors were obtained in both elutions from DNA-cellulose columns; these eluted, respectively, at 140–150 mM NaCl (lower-salt) and 180–190 mM NaCl (higher-salt). Therefore, the two major peaks detectable with extracts of mouse kidney result from differences that are intrinsic to the receptor complexes rather than heterogeneity of the DNA-cellulose matrix. These findings are discussed in relation to two forms of androgen receptors that exist in a different proportion in extracts of brain and in relation to other steroid receptors.

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