Affordable Access

Publisher Website

Chiral separation of β-methyl-amino acids by ligand exchange using capillary electrophoresis and HPLC

Authors
Journal
Journal of Pharmaceutical and Biomedical Analysis
0731-7085
Publisher
Elsevier
Publication Date
Volume
27
Identifiers
DOI: 10.1016/s0731-7085(01)00616-1
Keywords
  • β-Methyl-Amino Acids
  • Capillary Zone Electrophoresis
  • Enantiomer Separation
  • High Performance Liquid Chromatography
  • Ligand-Exchange
Disciplines
  • Chemistry

Abstract

Abstract This paper deals with the chiral separation of optical isomers of β-methyl-amino acids by CE and HPLC using the principle of ligand-exchange. Capillary zone electrophoresis was carried out using Cu(II) complexes of l-4-hydroxyproline ( l-4-Hypro), N-(2-hydroxypropyl)- l-4-hydroxyproline (HP- l-4-Hypro) and N-(2-hydroxyoctyl)- l-4-hydroxyproline (HO- l-4-Hypro) as chiral selectors, added to the electrolyte. The HPLC separations were performed on a chiral stationary ligand-exchange chromatography phase containing l-4-Hypro chemically bonded to silica gel. With both techniques nearly all compounds investigated are baseline resolved using different background electrolytes and mobile phases, respectively.

There are no comments yet on this publication. Be the first to share your thoughts.