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Protain Kinase C as a Biomarker for Assessing the Effect of Environmental Stress and Fungal Invasion on Plant Defense Mecanism

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  • Biology
  • Chemistry
  • Ecology
  • Geography
  • Medicine


植物には, 環境ストレスや病原菌感染に対する独自の抵抗性機構が備えられていると考えられている.我々は, 情報伝達系物質であるGタンパク質, フォスフォリパーゼC(PLC), プロテインキナーゼC(PKC)の量変化を調べることにより, 環境ストレスに対するバイオマーカーとしてのテストを行なった.モデル植物としてイネを使用し, ウエスタンブロッテング法を用いた.その結果, 様々な環境ストレスに対してPKCが最も敏感に反応し, また感受性も非常に高いバイオマーカーであることが判明した.PKCの量は除草剤, 低濃度の銅イオンの存在により増加した.また, 茎を折るなどの物理的処理をした場合においても同様の傾向がみられた.一方, 強風や高濃度の銅イオンではPKCレベルの減少がみられた.PKCレベルは, 病原菌(いもち病菌)をイネに接種した時に顕著に増加した.また, いもち病に対する非殺菌性防除剤であるプロベナゾール(PBZ)を処理した場合もPKCレベルは増加した. Plants are known to activate specific defense mechanisms in response to invasion by pathogens and environmental stresses. We examined the possibility of utilizing the changes in the amounts of protein kinase C (PKC) and its associated components of this signal transduction pathway as a biomarker of exposure of plants to the stress factors, using young rice plants as a model and a Western blotting method as the experimental tool. Preliminary studies have shown that PKC is a consistently more sensitive marker of exposure to a variety of environmental stress factors as compared to phospholipase C (PLC) or G-protein. The titer of PKC increased as a result of exposure to herbicides, low concentrations of copper, fungicides and other chemicals. The same trend was observed when rice plants were stressed by severe physical treatment such as broken stems, deprivation of sunlight and transplantation. On the other hand, PKC levels decreased upon exposure to high winds and high concentration of copper. The most drastic rise in PKC was observed when rice plants were inoculated with the rice blast fungus Pyricularia oryzae. As expected, several plant protectants against the fungal invasion also induce a rise in PKC.

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