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Somatic Embryogenesis from Cultured Leaf Explants of the Tropical TreeEuphoria longanStend.

Authors
Journal
Journal of Plant Physiology
0176-1617
Publisher
Elsevier
Publication Date
Volume
132
Issue
2
Identifiers
DOI: 10.1016/s0176-1617(88)80159-7
Keywords
  • Euphoria Longan
  • Somatic Embryogenesis
  • Callus

Abstract

Summary Embryogenic callus was induced from leaflets of new vegetative flushes of mature longan ( Euphoria longan Stend.) trees. Induction of embryogenic competency was dependent on the presence of auxin (2,4-D) and cytokinin (kinetin) in the growth medium (B5 with 400mg liter- 1 glutamine, 60g liter- 1 sucrose, 200 mg liter- 1 casein hydrolysate and 1.7g liter- 1 Gelrite). The optimum range of phytohormone concentrations was 0.25 - 2.0 mg liter- 1 of kinetin and 2,4-D in every combination. Somatic embryos developed to maturity on B5 medium containing glutamine, casein hydrolysate, 20 g liter- 1 sucrose, 10% (v/v) coconut water and 1.7 g liter- 1 Gelrite. Callus initiation and somatic embryo development occurred in darkness at 25°C. Mature somatic embryos germinated normally in the light.

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