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Degradation of nuclear proteins: studies on transplanted B82 cell karyoplast proteins

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
210
Issue
2
Identifiers
DOI: 10.1016/0014-5793(87)81329-7
Keywords
  • Karyoplast
  • Protein Degradation
  • Cell Nucleus
  • Lysosome
  • (L Cell)
Disciplines
  • Biology

Abstract

Abstract Karyoplasts were prepared from B82 cells (thymidine kinase deficient mouse L cells) by cytochalasin B mediated enucleation. Morphological measurements show that the nucleus constitutes 89% of a karyoplast by volume. Homokaryons were obtained by Sendai virus mediated karyoplast-B82 cell fusion. Transplanted nuclei were not destroyed catastrophically but were maintained intracellularly for at least 140 h. Transplanted nuclear proteins were degraded with an average half-life of 84 ± 7 h by processes partially sensitive to inhibition by NH 4Cl (50%) and leupeptin (30%). The data therefore suggest that some nuclear proteins are translocated to the cytoplasm for lysosomal degradation.

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