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Antioxidant status and dietary lipid unsaturation modulate oxidative DNA damage

Authors
Journal
Free Radical Biology and Medicine
0891-5849
Publisher
Elsevier
Publication Date
Volume
16
Issue
1
Identifiers
DOI: 10.1016/0891-5849(94)90247-x
Keywords
  • Antioxidants
  • 8-Hydroxy-2′-Deoxyguanosine
  • Free Radicals
  • Lipid Peroxidation
  • Cancer
  • Hplc-Ec
Disciplines
  • Chemistry

Abstract

Abstract High performance liquid chromatography with electrochemical detection (HPLC-EC) was used to measure 8-hydroxy-2′-deoxyguanosine (8-OHdG), a marker for oxidative DNA damage, in mammary gland isolated from tumor-bearing and tumor-free rats fed diets of varied fatty acid composition and vitamin E and selenium content. A method for tissue preparation and analysis is reported and a significant positive correlation shown between degree of unsaturation of dietary fatty acids and 8-OHdG concentration, regardless of antioxidant status. The increase in 8-OHdG concentration with greater fatty acid unsaturation was more pronounced in the absence of adequate dietary vitamin E and selenium. The implications of these data for defining the role of dietary lipid in the process of mammary carcinogenesis are discussed.

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