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Dilution effect of carbonate ion on isotachophoretic zones observed with scanning UV photometric detection

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
555
Identifiers
DOI: 10.1016/s0021-9673(01)87185-5
Disciplines
  • Biology

Abstract

Abstract A problem in the isotachophoretic analysis of anions at high pH is the so-called ‘dilution effect’ originating from aerial carbon dioxide dissolved in the terminating electrolyte. Elongation of isotachophoretic zones due to this dilution effect was studied with a scanning UV photometric detector, varying the carbonate concentration in the terminating electrolyte from 0 to 3 m M. The zone of a component with a smaller effective mobility than that of carbonate ion was elongated in proportion to the carbonate concentration, and the present system was still isotachophoretic, although it had two criteria of migration velocity. A practical example of the dilution effect in protein analysis at high pH using a model mixture of globulin, transferin and albumin is presented.

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