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On the generating factor of nonlinea dielectric responses of Saccharomyces cerevisiae

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  • Nonlinear Dielectric Property / Biological Activity / Membrane Filter / Electrode
  • Biology
  • Chemistry
  • Musicology


Mem. Fac. Eng., Osaka City Univ., Vol. 43, pp.13-17 (2002) On the generating factor of nonlinear dielectric responses of Sa.ccharomyces cerevisiae Tsutomu INUISHI *, Masafumi MURAJI** and Hiroaki TSUJIMOTO *** (Received October 15, 2002) Synopsis The harmonics of response wave based on the nonlinear dielectric properties of yeast cells have particular patterns for biological activity. The method is a novel technique for determining the activity of living cells. In this paper, we examined the generating factor of the nonlinear dielectric responses by comparing the results in the case where yeast cells exist near the electrodes of an electrochemical cell with those in the case where yeast cells do not. It was ascertained that nonlinear dielectric responses were induced according to biological activities. KEYWARDS: Nonlinear dielectric property, Biological activity, Membrane filter, Electrode 1. Introduction When an ac voltage is applied to cell suspensions and response wave (electric current wave from cell suspension) is analyzed using FFT, the harmonics of the response wave show peculiar patterns according to biological activities. It is considered that the electric current wave is effected by the biological activity of cell. This phenomenon is called nonlinear dielectric property(l). It is thought that these differences in the responses according to cell activity are derived from the membrane proteins and are mainly ascribable to by the H+-ATPase in the plasma membrane. H. Yositake et a1 studied nonlinear dielectric properties of yeast cells using 2-electrodes system(2) in which the responses are easier to be influenced by adhesion of yeast cells on electrodes than 4-electrodes system. We examine generating factor of nonlinear dielectric responses by using 2-electrodes system. First we measured nonlinear dielectric responses in each growth phase (2h, 7h, 25h) by using 2-electrode system. Then, to examine the effect of yeast adhesion on electrode surface, we produced the electroc

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