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Determination of the Relative Percentage Deuteration of the Ribosomal Protein S4 by Electrospray Ionization Mass Spectrometry

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
308
Issue
1
Identifiers
DOI: 10.1006/abbi.1994.1003
Disciplines
  • Biology
  • Economics

Abstract

Abstract Electrospray ionization mass spectrometry provides a fast, economical, and accurate new method for determining levels of nonexchangeable deuterium incorporation in proteins derived from organisms grown on deuterated media. Results are shown for determining the percentage deuteration of ribosomal protein S4 from Escherichia coli.

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