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Structural studies on the polar glycoinositol phospholipids ofTrypanosoma(Schizotrypanum)dionisiifrom bats

Authors
Journal
Molecular and Biochemical Parasitology
0166-6851
Publisher
Elsevier
Publication Date
Volume
102
Issue
1
Identifiers
DOI: 10.1016/s0166-6851(99)00107-3
Keywords
  • Electrospray
  • Glycoinositol Phospholipids
  • Glycosylphosphatidylinositol
  • Inositol-Phosphoceramide
  • Schizotrypanum
  • Trypanosomatidae
Disciplines
  • Chemistry

Abstract

Abstract The polar glycoinositol phospholipids (GIPLs) of a Trypanosoma species that belongs to the Schizotrypanum subgenus were purified by reversed-phase and normal-phase liquid chromatography and analysed by negative-ion mode electrospray-mass spectrometry (ES-MS). The phosphatidylinositol moieties were released by nitrous acid deamination and identified as ceramide- and alkylacylglycerol-containing species. The structures of the GIPLs were determined using chemical treatments, sequential exoglycosidase digestions and positive-ion mode ES-MS-MS. All of the GIPLs were based on the same Manα1-2Manα1-2Manα1-6Manα1-4(NH 2-CH 2CH 2-HPO 3-)GlcN-PI core with single terminal Gal f residue substitutions either on the terminal nonreducing Man or on the second αMan residue from the inositol and with either ethanolamine phosphate or 2-aminoethylphosphonate on the third αMan residue from the inositol. The T. ( S.) dionisii GIPLs are compared with those of T. ( S.) cruzi, a closely related species of the Schizotrypanum subgenus.

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