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Transformation ofMycobacterium aurumby electroporation: the use of glycine, lysozyme and isonicotinic acid hydrazide in enhancing transformation efficiency

Authors
Journal
FEMS Microbiology Letters
0378-1097
Publisher
Oxford University Press
Publication Date
Volume
72
Identifiers
DOI: 10.1016/0378-1097(90)90375-z
Keywords
  • Transformation Efficiency
  • Electroporation

Abstract

Abstract A transformation procedure for Myobacterium aurum using electroporation was developed and optimized. Effects of glycine and lysozyme treatments were studied, and isonicotinic acid hydrazide was shown to increase transformation efficiency ten-fold.

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