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Nigrosin as a dye for differentiating live and dead ascites cells

Authors
Journal
Experimental Cell Research
0014-4827
Publisher
Elsevier
Publication Date
Volume
15
Issue
1
Identifiers
DOI: 10.1016/0014-4827(58)90067-3

Abstract

Abstract The application of nigrosin as a stain for differentiating between live cells with unchanged membranes and cells whose membranes have been modified either experimentally or by death of the cell has been described. The advantages of nigrosin have been shown to be its high contrast between stained and unstained cells, and its non-toxic nature. A simple method is given for modifying ascites cells, i.e., modifying the cell membrane so that it becomes permeable to large molecules such as nigrosin. The ability of modified cells to metabolize is briefly discussed.

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