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The chromosome complement ofErysiphe graminisf.sp.hordeianalysed by light microscopy and field inversion gel electrophoresis

Authors
Journal
Mycological Research
0953-7562
Publisher
Elsevier
Publication Date
Volume
96
Issue
2
Identifiers
DOI: 10.1016/s0953-7562(09)80922-2

Abstract

Light microscopy studies of somatic barley powdery mildew fungal cells at metaphase revealed seven to eight chromosomes of at least three different sizes. Separation of fungal chromosomal DNA by field inversion gel electrophoresis (FIGE) was achieved using a combination of very short and long pulses. FIGE showed distinct bands representing chromosomal DNA, mitochondrial DNA and extrachromosomal plasmid DNA. By combining the results from the two techniques used, the haploid genome of the barley powdery mildew fungus is found to contain five very small chromosomes (0·2–0·8 Mb) and a group of at least two larger chromosomes.

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