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A DNA vaccine coding for the chimera BLSOmp31 induced a better degree of protection againstB. ovisand a similar degree of protection againstB. melitensisthanRev.1vaccination

Authors
Journal
Vaccine
0264-410X
Publisher
Elsevier
Publication Date
Volume
25
Issue
32
Identifiers
DOI: 10.1016/j.vaccine.2007.05.049
Keywords
  • Brucella Spp.
  • Vaccine
  • Dna Vaccine
  • Outer Membrane Protein
  • Virus Like Particule
Disciplines
  • Biology

Abstract

Abstract In the present study, we reported an attempt to improve the immunogenicity and protective capacity of the chimera BLSOmp31 using a different antigen delivery: DNA vaccination. Vaccination of BALB/c mice with the DNA vaccine coding for the chimera BLSOmp31 (pCIBLSOmp31) provided the best protection level against Brucella ovis, which was significantly higher than the given by the co-delivery of both plasmids coding for the whole proteins (pcDNABLS + pCIOmp31) and even higher than the control vaccine Rev.1. Moreover, pCIBLSOmp31 induced higher protection against Brucella melitensis than pcDNABLS + pCIOmp31 but similar protection than Rev.1. The chimera induced a strong humoral response against the inserted peptide. It also induced peptide- and BLS-specific cytotoxic T responses. The insertion of this peptide on BLS induced stronger T helper 1 responses specific for the carrier (BLS), thus our results represent a case of synergic strengthening between two Brucella antigens. Hitherto, this is the first indication that a recombinant subunit vaccine elicits greater protection than whole Brucella.

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