Affordable Access

Publisher Website

Isolation of a neurotoxin with a presynaptic action from the venom of the black widow spider (Latrodectus mactans, Fabr.)

Authors
Journal
Toxicon
0041-0101
Publisher
Elsevier
Publication Date
Volume
14
Issue
4
Identifiers
DOI: 10.1016/0041-0101(76)90030-1
Disciplines
  • Biology

Abstract

Abstract Whole venom gland homogenates from the black widow spider were fractionated by discontinuous polyacrylamide gel electrophoresis. Eleven fractions were extracted from gel sections and assayed for toxicity by injection into cockroach nymphs and by application to cockroach neuromuscular preparation. One of the fractions, a slowly migrating protein with a molecular weight of 125,000, produced a slow permanent paralysis and accounted for the major action of crude venom by inducing a large transient rise in the miniature endplate potential frequency and eventual synaptic failure. A possible second neurotoxin with a different action has also been isolated.

There are no comments yet on this publication. Be the first to share your thoughts.