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Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting

Authors
Publisher
American Society of Plant Biologists
Publication Date
Source
PMC
Keywords
  • Research Articles
Disciplines
  • Mathematics

Abstract

Receptor Salvage from the Prevacuolar Compartment Is Essential for Efficient Vacuolar Protein Targeting by Luis L.P. daSilva, J. Philip Taylor, Jane L. Hadlington, Sally L. Hanton, Christopher J. Snowden, Sarah J. Fox, Ombretta Foresti, Federica Brandizzi, and Jürgen Denecke Plantcell Volume 17(1):132-148 January 3, 2005 ©2005 by American Society of Plant Biologists Wortmannin Strongly Inhibits the Sequence-Specific Vacuolar Sorting Route.(A) The concentration-dependent influence of the drug wortmannin on the secretion index of amy (white bars) and amy-spo (gray bars) in tobacco mesophyl protoplasts. daSilva L et al. Plantcell 2005;17:132-148 ©2005 by American Society of Plant Biologists Confirmation of amy-spo as a BP80-Ligand in Vivo.(A) Transport assay for soluble BP80 molecules with or without the ER retention motif HDEL via transient expression in tobacco protoplasts. daSilva L et al. Plantcell 2005;17:132-148 ©2005 by American Society of Plant Biologists Wortmannin Does Not Prevent BP80-Ligand Interactions.(A) Wortmannin does not prevent BP80 from physically interacting with its ligands. daSilva L et al. Plantcell 2005;17:132-148 ©2005 by American Society of Plant Biologists Truncated BP80 Lacking the Ligand Binding Domain Interferes with Vacuolar Sorting.(A) Schematic illustration of the nature and topology of the truncated BP80 fusions in relation to the full-length BP80. daSilva L et al. Plantcell 2005;17:132-148 ©2005 by American Society of Plant Biologists Truncated BP80 Is Codominant versus Full-Length BP80.(A) The concentration-dependent influence of GFP-BP80 coexpression on the secretion index of amy and amy-spo in tobacco mesophyl protoplasts. daSilva L et al. Plantcell 2005;17:132-148 ©2005 by American Society of Plant Biologists Confocal Laser Scanning Microscopy Demonstrating Increased Vacuolar Sorting of GFP-BP80 by Wortmannin.(A) Protoplasts were cotransfected with 10 μg of plasmids encoding GFP-BP80a and the Golgi marker ST-YFP, live-embedded in TEX medium w

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