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Characterization of DNA adenine methylation mutants ofEscherichia coliK12

Authors
Journal
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
0027-5107
Publisher
Elsevier
Publication Date
Volume
59
Issue
2
Identifiers
DOI: 10.1016/0027-5107(79)90153-2
Disciplines
  • Physics

Abstract

Abstract The phenotypic traits of 7 independently isolated dam mutants of Escherichia coli have been examined. The mutant strains differ from the wildtype in the following respects: (1) decreased DNA adenine methylase activity in vivo and in vitro; (2) a 14–85-fold increase in spontaneous mutability; (3) decreased survival after ultraviolet irradiation; (4) a 10–21-fold increase in spontaneous induction of λ phage from lysogens; (5) a 3–17-fold increase in the level of recombination; and (6) inviability of double mutants containing dam − and 3itrecB − or recC −. Unmethylated fd phage chromosomes are able to replicate normally in dam − mutants. A mutant strain in which the dcm gene is deleted is viable, showing that the dcm gene product is dispensible for growth.

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