Palladin is a phosphoprotein with a role in regulating the actin cytoskeleton. Recently, palladin was identified as being overexpressed and mutated in a form of familial pancreatic cancer, an extremely lethal disease. Protein expression studies indicated that palladin was overexpressed in the tumor cells, but immunohistochemistry data from a separate study showed that palladin was actually overexpressed in the non-neoplastic stroma of a pancreatic tumor. The goal of this study was to determine which cell type overexpressed palladin using various palladin antibodies. We found that the fibroblasts in the stroma of a pancreatic tumor overexpressed multiple isoforms of palladin. The palladin expression occurs in a population of fibroblasts in the tumor microenvironment known as tumor-associated fibroblasts. These cells resemble the fibroblasts present in healing wounds, a cell type where palladin is also expressed. The consequence of palladin upregulation in these cells in the context of a pancreatic tumor is unknown.