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Crystallization and preliminary X-ray diffraction analysis of Q4DV70 from Trypanosoma cruzi, a hypothetical protein with a putative thioredoxin domain

Authors
Journal
Acta Crystallographica Section F Structural Biology and Crystallization Communications
1744-3091
Publisher
International Union of Crystallography
Publication Date
Volume
65
Issue
6
Identifiers
DOI: 10.1107/s1744309109017734
Keywords
  • Crystallization Communications
Disciplines
  • Biology

Abstract

The gene coding for Q4DV70 has been cloned and the protein overexpressed in Escherichia coli with an N-­terminal His tag. Purification of Q4DV70 was carried out by affinity and size-exclusion chromatography and the His tag was removed by TEV protease digestion. Crystals of Q4DV70 were grown using the sitting-drop vapour-diffusion method.

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