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An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA.

Authors
Type
Published Article
Journal
Nucleic Acids Research
0305-1048
Publisher
Oxford University Press
Volume
37
Issue
8
Pages
2757–2770
Identifiers
DOI: 10.1093/nar/gkp068
Source
Kowalczykowski Lab

Abstract

Rad54 protein is a key member of the RAD52 epistasis group required for homologous recombination in eukaryotes. Rad54 is a duplex DNA translocase that remodels both DNA and protein-DNA complexes, and functions at multiple steps in the recombination process. Here we use biochemical criteria to demonstrate the existence of this important protein in a prokaryotic organism. The Sulfolobus solfataricus Rad54 (SsoRad54) protein is a double-strand DNA-dependent ATPase that can alter the topology of duplex DNA. Like its eukaryotic homolog, it interacts directly with the S. solfataricus Rad51 homologue, SsoRadA, to stimulate DNA strand exchange. Confirmation of this protein as an authentic Rad54 homolog establishes an essential phylogenetic bridge for identifying Rad54 homologs in the archaeal and bacterial domains.

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