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Liquid chromatography–mass spectrometry analysis of hydroxylated polycyclic aromatic hydrocarbons, formed in a simulator of the human gastrointestinal tract

Authors
Journal
Journal of Chromatography B
1570-0232
Publisher
Elsevier
Publication Date
Volume
806
Issue
2
Identifiers
DOI: 10.1016/j.jchromb.2004.04.001
Keywords
  • Polynuclear Aromatic Hydrocarbons

Abstract

Abstract Described is a liquid chromatography–mass spectrometry (LC–MS) procedure for the determination of hydroxylated biotransformation products of polycyclic aromatic hydrocarbons (PAH) in the human gastrointestinal tract. The formation of hydroxylated PAHs was monitored upon incubation of PAHs with colon microbiota from the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). The analytical method consisted of a biomass removal step followed by a solid phase extraction (SPE) step using C18 packed columns to remove non-digested food compounds and microbial metabolites that interfere with the detection of the target compounds. For quantification, 9-hydroxyphenanthrene 13 C 6 was used as the internal standard. The detection limits of the hydroxylated PAHs were generally in the range 0.36–14.09 μg l −1, based on a signal/noise ratio of 3:1. The recovery of hydroxylated PAHs in intestinal suspension was variable ranging from 45 to 107%, with relative standard deviation (R.S.D.) between 5 and 17%. The analytical procedure was used to show the microbial production of 1-hydroxypyrene and 7-hydroxybenzo( a)pyrene, metabolites that may give colon incubated PAHs bioactive properties.

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