Affordable Access

Publisher Website

Increasing mouse embryonic fibroblast cells adhesion on superhydrophilic vertically aligned carbon nanotube films

Authors
Journal
Materials Science and Engineering C
0928-4931
Publisher
Elsevier
Publication Date
Volume
31
Issue
7
Identifiers
DOI: 10.1016/j.msec.2011.06.007
Keywords
  • Vacnts
  • Oxygen Plasma
  • Wettability
  • Superhydrophilic
  • Cell Adhesion
Disciplines
  • Biology
  • Chemistry
  • Physics

Abstract

Abstract We have analyzed the adhesion of mouse embryonic fibroblasts (MEFs) genetically modified by green fluorescence protein (GFP) gene cultured on vertically-aligned carbon nanotubes (VACNTs) after 6 days. The VACNTs films grown on Ti were obtained by microwave plasma chemical vapor deposition process using Fe catalyst and submitted to an oxygen plasma treatment, for 2 min, at 400 V and 80 mTorr, to convert them to superhydrophilic. Cellular adhesion and morphology were analyzed by scanning electron, fluorescence microscopy, and thermodynamics analysis. Characterizations of superhydrophilic VACNTs films were evaluated by contact angle and X-Ray Photoelectron Spectroscopy. Differences of crowd adhered cells, as well as their spreading on superhydrophilic VACNTs scaffolds, were evaluated using focal adhesion analysis. This study was the first to demonstrate, in real time, that the wettability of VACNTs scaffolds might have enhanced and differential adherence patterns to the MEF-GFP on VACNTs substrates.

There are no comments yet on this publication. Be the first to share your thoughts.