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The biosynthesis of thiamine conversion of 2-methyl-4-amino-5-formylpyrimidine to 2-methyl-4-amino-5-hydroyxmethylpyrimidine by cell-free extracts of baker's yeast

Authors
Journal
Biochimica et Biophysica Acta (BBA) - General Subjects
0304-4165
Publisher
Elsevier
Publication Date
Volume
201
Issue
2
Identifiers
DOI: 10.1016/0304-4165(70)90308-9
Disciplines
  • Biology
  • Medicine

Abstract

Abstract 1. 1. Cell-free preparations of Saccharomyces cerevisiae, were shown to catalyze the reduction of 2-methyl-4-amino-5-formylpyrimidine to the corresponding 5-hydroxymethyl derivative, a known intermediate in thiamine biosynthesis. Either NADH or NADPH served as a cofactor. The pH optimum of the system was 6.5–7.0 and the apparent K m for the formylpyrimidine substrate was 3.3·10 −4 M. 2. 2. Details of a microbiological assay for the pyrimidine moiety of thiamine, using Escherichia coli Strain M70-17, are described.

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